Aberrations in 4Pi Microscopy
نویسندگان
چکیده
منابع مشابه
4Pi spectral self-interference microscopy.
Spectral self-interference microscopy (SSM) relies on the balanced collection of light traveling two different paths from the sample to the detector, one direct and the other indirect from a reflecting substrate. The resulting spectral interference effects allow nanometer-scale axial localization of isolated emitters. To produce spectral fringes the difference between the two optical paths must...
متن کامل4Pi microscopy with negligible sidelobes
The coherent addition of the wavefronts of two opposing highangle lenses provides an axial (z) resolution improvement by 5–7-fold in farfield fluorescence microscopy. However, all microscopy concepts based on this principle have so far required mathematical deconvolution of the acquired data. This stems from the fact that the decrease of the axial width of the effective point spread function (E...
متن کامل4Pi-Confocal Microscopy Is Coming of Age
Fluorescence microscopy is one of the most favored imaging techniques in biological research: It allows non-invasive imaging of sub-cellular structures inside of live cells, specifically labeled by biological markers such as the green fluorescent protein (GFP). The confocal laser-scanning microscope or its multiphoton versions stand out by their ability to provide 3D-images. However, like any o...
متن کامل4pi-microscopy with 1-photon Excitation
Over the last years two-photon excitation 4Pi-microscopy with an axial resolution of ~100 nm has been demonstrated in multiple biological applications [1-3]. The artefact-free presentation of the recorded structures has been possible because of the contiguous optical transfer function (OTF), which allows reliable image deconvolution. The use of two-photon excitation (2PE) has been critical to s...
متن کامل4Pi confocal microscopy with alternate interference.
We introduce 4Pi confocal microscopy with destructive interference of converging waves. Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. Three-dimensional imaging of microtubules of a mouse...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optics Express
سال: 2017
ISSN: 1094-4087
DOI: 10.1364/oe.25.014049